Comparing Effects of: Blastema of Axolotl, Platelet Rich Plasma of Axolotl and Platelet Rich Plasma of Rat on Scalded Injury
Introduction: Burns are commonly seen in children and the most common cause is scalding. Therefore, the aim is to obtain the "ideal care product" and "ideal burn treatment" in the burn. Wound healing of the axolotls is scarless and their tissues regeneration is perfect. This suggests that axolotls can be effective in the healing of burns. For this purpose, Platelet Rich Plasma (PRP) and its efficacy have not been established previously and blastema tissue which is important in the renewal of axolotls was investigated.
In our study, we wanted to investigate the efficacy of Axolotls Blastema and PRP.
Material and Method: 40 rats were used for the burn model. 10 "Ambystoma Mexicanum" type axolotl were used for Blastema and PRP production. The extremities of the axolotls were amputated to obtain blastema 7 days before burn. PRP was prepared by the same axolotls. Rat PRP was prepared with the same protocol, too.
In all rats, scalded burn was formed by contacting 100 Â°C water for 10 seconds and randomly divided into 5 groups.
- Group-Control; no application was made
- Group-Sham; topical silver sulfadiazine was applied
- Group–Rat PRP; PRP injected
- Group-Axolotl PRP; PRP injected
- Group -Axolotl Blastema; Blastema injected
Macroscopic evaluation was performed with "Image J" program. For microscopic evaluation, "distance measurement between wound" and "scores" were performed.
Immunohistochemical evaluation revealed with VEGF-alpha, IL-6, TNF-alpha and Collagen-I values in the tissue.
Discussion: In macroscopic evaluation; the highest decrease (2,84 cm2) and the highest score (10,8) was detected in the blastema group. The "closure distance" was shortest in the Blastema Group (644,133 μm). All cytokines in the immunohistochemical evaluation were found in favor of blastema group. Epithelialization was very rapid in the blastema group.
Result: It is the first study in the literature also in the plastic surgery where the tissues and cells of the Axolotls are used in the mammals. Axolotl PRP and Axolotl Blastema were first investigated in the treatment of burn. The best result in burn healing was seen in the blastema group; this result is statistically significant.
Blastema and PRP obtainment protocols are described. The new protocols we have created for "Obtaining Axolotl Blastema", "Obtaining Axolotl PRP" and "Standard Burn Formation Model"; it can also be used effectively in other studies.
With the help of these methods, we believe that new products can be developed to reduce burn recovery time, cost and hospital stay. We think that axolotl welded product can be obtained for burn, nerve healing and tissue regeneration with advanced researches.